OpenWetWare is a wiki whose mission is "to support open research, education, publication, and discussion in biological sciences and engineering."
OpenWetWare was created by graduate students at MIT on April 20, 2005. Initially, it served as a private lab wiki for the labs of Drew Endy and Tom Knight at MIT. The site was opened up to allow any lab to join on June 22, 2005. As of April 6, 2007 the site hosted 100 research laboratories from over 40 institutions, including Boston University, Brown University, Caltech, Cambridge Research Institute, CNRS, Duke University, and many others.
In addition to laboratories, a number of scientific communities are based on the site, including synthetic biology, Mimulus, and the BioBricks Foundation. One scientific community is the iGEM community with over 60 different teams represented on June 28, 2013 including the | NRP-UEA-Norwich team and the | Groningen team.
OpenWetWare runs on MediaWiki software on Linux servers. All content is available under free content licenses, specifically the GNU free documentation license (GFDL) and the Creative Commons Attribution ShareAlike license.
The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein assay or biuret reagent. The total protein concentration is exhibited by a color change of the sample solution from green to purple in proportion to protein concentration, which can then be measured using colorimetric techniques.Biometrology
Biometrology refers to measurement and data activities that provide quantitative characterization of biology. Biometrology advances laboratory techniques regarding scalable bioproducts or services.Bionumbers
BioNumbers is a free-access database of quantitative data in biology designed to provide the scientific community with access to the large amount of data now generated in the biological literature. This aims to make quantitative values more easily available, to aid fields such as systems biology.
The BioNumbers project performs literature-based curation of various sources. It is a regularly updated online resource that contains >13,000 entries from ~1,000 distinct references. Examples of data include transcription and translation rates, organism and organelle sizes, metabolites concentrations and growth rates. Entries are provided with full reference and details such as measurement method and comments. BioNumbers also publishes a monthly review of a problem in quantitative biology.Bromophenol blue
Bromophenol blue (3′,3″,5′,5″-tetrabromophenolsulfonphthalein, BPB, albutest) is used as a pH indicator, a color marker, and a dye. It can be prepared by slowly adding excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid.Cresol Red
Cresol red (full name: o-cresolsulfonephthalein) is a triarylmethane dye frequently used for monitoring the pH in aquaria.Drew Endy
Andrew (Drew) David Endy (born 1970) is a synthetic biologist and Tenured Professor of bioengineering at Stanford University, California.MOPS
MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES. Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.Manchester Institute of Biotechnology
The Manchester Institute of Biotechnology, formerly the Manchester Interdisciplinary Biocentre (MIB) is a research institute of the University of Manchester, England. The centre has been designed to enable academic communities to explore specific areas of interdisciplinary quantitative bioscience, largely through the efforts of multidisciplinary research teams. Research at MIB follows three broadly defined, interdisciplinary and complementary themes: Biological Mechanism and Catalysis, Molecular Bioengineering, Systems biology.Planning for the institute began late in 1998 and culminated with the official opening on 25 October 2006 of the John Garside Building. The building won "Building of the Year" from Manchester Chamber's Building and Development Committee in 2006 along with Beetham Tower, Manchester.
The building has featured in several television commercials, notably Injury Lawyers 4u.The institute was renamed the Manchester Institute of Biotechnology on 1 June 2012, retaining the acronym MIB.Medicines for Malaria Venture
Medicines for Malaria Venture (MMV), a not-for-profit public-private partnership, was established as a foundation in Switzerland in 1999. Its mission is to reduce the burden of malaria in disease-endemic countries by discovering, developing and facilitating delivery of new, effective and affordable antimalarial drugs. Its vision is a world in which these innovative medicines will cure and protect the vulnerable and under-served populations at risk of malaria, and help to ultimately eradicate this terrible disease.Open-door academic policy
An open-door academic policy, or open-door policy, is a policy if a university accepting to enroll students without asking for evidence of previous education, experience, or references. Usually, payment of the academic fees (or financial support) is all that is required to enroll.
Universities may not employ the open-door policy for all their courses, and those that have a universal open-door policy where all courses have no entry requirements are called open universities. The policy is seen to be a part of the educational revolution. From the dictionary meaning of the open-door policy, which is the idea of granting access to those who want access to the country freely, a similar idea can be drawn in terms of education.According to Deepa Rao, the open-door academic policy is one of the main ways in which adult learners become a part of university/college life. The recognized demand for post-secondary education made many institutions commit strongly to the policy, but many concealed limitations in the policy can prevent some from securing a degree.Open admissions
Open admissions, or open enrollment, is a type of unselective and noncompetitive college admissions process in the United States in which the only criterion for entrance is a high school diploma or a certificate of attendance or General Educational Development (GED) certificate.Open university
An open university is a university with an open-door academic policy, with minimal or no entry requirements. Open universities may employ specific teaching methods, such as open supported learning or distance education. However, not all open universities focus on distance education, nor do distance-education universities necessarily have open admission policies.P2P Foundation
P2P Foundation: The Foundation for Peer to Peer Alternatives is an organization with the aim of studying the impact of peer to peer technology and thought on society. It was founded by Michel Bauwens, James Burke and Brice Le Blévennec.The P2P Foundation is a registered institute founded in Amsterdam, Netherlands. Its local registered name is: Stichting Peer to Peer Alternatives, dossier nr: 34264847.Science 2.0
Science 2.0 is a suggested new approach to science that uses information-sharing and collaboration made possible by network technologies. It is similar to the open research and open science movements and is inspired by Web 2.0 technologies. Science 2.0 stresses the benefits of increased collaboration between scientists. Science 2.0 uses collaborative tools like wikis, blogs and video journals to share findings, raw data and "nascent theories" online. Science 2.0 benefits from openness and sharing, regarding papers and research ideas and partial solutions.A general view is that Science 2.0 is gaining traction with websites beginning to proliferate, yet at the same time there is considerable resistance within the scientific community about aspects of the transition as well as discussion about what, exactly, the term means. There are several views that there is a "sea change" happening in the status quo of scientific publishing, and substantive change regarding how scientists share research data. There is considerable discussion in the scientific community about whether scientists should embrace the model and exactly how Science 2.0 might work, as well as several reports that many scientists are slow to embrace collaborative methods and are somewhat "inhibited and slow to adopt a lot of online tools."Scientific journal
In academic publishing, a scientific journal is a periodical publication intended to further the progress of science, usually by reporting new research.Southern blot
A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.
The method is named after the British biologist Edwin Southern, who first published it in 1975. Other blotting methods (i.e., western blot, northern blot, eastern blot, southwestern blot) that employ similar principles, but using RNA or protein, have later been named in reference to Edwin Southern's name. As the label is eponymous, Southern is capitalised, as is conventional for proper nouns. The names for other blotting methods may follow this convention, by analogy.T7 RNA polymerase
T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA from DNA in the 5'→ 3' direction.Vector NTI
Vector NTI is a commercial bioinformatics software package used by many life scientists to work, among other things, with nucleic acids and proteins in silico . It allows researchers to, for example, plan a DNA cloning experiment on the computer before actually performing it in the lab.
It was originally created by InforMax Inc, North Bethesda, MD. Initially released for free, it was locked and turned into a commercial software after 2008 which created problems for locked in users who were forced to buy the software to continue accessing their data on newer computers . It also caused many scientists to search for alternatives .
What was previously a single software package has been split into Vector NTI Express, Advanced, and Express Designer .Xylene cyanol
Xylene cyanol can be used as a color marker, or tracking dye, to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. Bromophenol blue and orange G can also be used for this purpose.
Once mixed with the sample, the concentration of xylene cyanol is typically about 0.005% to 0.03%.